In X. tropicalis, human, and mouse, the consensus NRSE motifs share sequence similarity and the majority of the NRSE sites are farther than 20 kb from a gene, with a single copy per gene. A. Sequence logos of the consensus NRSE motif derived from 32 bona fide mouse REST target genes (, top panel), from 1301 human NRSE motifs (, middle panel), and from 742 X. tropicalis NRSE motifs (bottom panel). Arrowheads mark the differences between the Xenopus and human consensus motifs. B. In all three vertebrate genomes, ~ 65% of the motifs are located farther than 20 kb from a gene. C. 80% to 90% of the putative REST target genes in three organisms have a single NRSE motif within 100 kb of a gene.