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Table 3 Differentially expressed proteins in both ex vivo models

From: Quantitative proteomic analysis of host—pathogen interactions: a study of Acinetobacter baumannii responses to host airways

Protein description AbH12O-A2 locus Sec.a BALF model/controlb BALF model/control p-valuec macrophage model/controlb macrophage model/control p-valuec
Pathogenesis and virulence
Protein CsuC AIS07073.1 YES 2.19 (0.76) 0.003 0.16 (0.06) 0.004
Protein CsuA AIS07075.1 YES 1.87 (0.95) 0.036 0.31 (0.22) 0.040
Membrane protein (OmpA) AIS07737.1 YES 1.51 (0.65) 0.015 7.80 (2.78) 0.049
Oligopeptidase A AIS07885.1 NO 0.48 (0.11) 0.001 4.83 (0.88) 0.006
ABC transporter ATP-binding protein (YjjK) AIS08062.1 NO 1.74 (0.61) 0.038 2.33 (0.77) 0.046
Amino acid metabolism and transport
Aspartate aminotransferase AIS07065.1 NO 0.08 (0.07) 0.000 0.39 (0.18) 0.010
Carbohydrate metabolism and transport
Phosphoglyceromutase AIS05033.1 NO 0.26 (0.14) 0.020 3.94 (1.06) 0.032
Cell wall/membrane/envelope biogenesis
UDP-N-acetylmuramate-alanine ligase (MurC) AIS08199.1 NO 99.08 (0.00) 0.020 8.17 (3.95) 0.040
Coenzyme metabolism
3-Phosphoglycerate dehydrogenase AIS07996.1 NO 0.38 (0.09) 0.016 3.77 (0.88) 0.014
Energy production and conversion
Isocitrate dehydrogenase AIS07371.1 NO 0.29 (0.05) 0.004 4.57 (0.55) 0.002
Acetyl-CoA hydrolase AIS08069.1 NO 2.05 (0.67) 0.008 4.74 (1.68) 0.040
Unknown function
Hypothetical protein AIS05481.1 YES 0.07 (0.10) 0.013 0.17 (0.08) 0.000
DNA-binding protein AIS07086.1 YES 0.08 (0.07) 0.006 0.24 (0.17) 0.037
Glyoxalase AIS07740.1 YES 0.39 (0.45) 0.015 0.30 (0.23) 0.036
Inorganic ion transport and metabolism
Hydroperoxidase AIS06129.1 YES 0.26 (0.10) 0.000 0.35 (0.13) 0.008
Lipid metabolism
Short-chain dehydrogenase AIS06130.1 YES 0.04 (0.05) 0.040 0.10 (0.07) 0.032
Nucleotide metabolism and transport
Deoxyuridine 5′-triphosphate nucleotidohydrolase AIS05628.1 NO 4.45 (6.37) 0.039 0.28 (0.32) 0.032
Transcription
DNA-directed RNA polymerase subunit beta’ AIS05081.1 NO 0.10 (0.04) 0.000 2.86 (0.42) 0.025
Translation
30S ribosomal protein S6 AIS07031.1 NO 3.56 (0.80) 0.004 6.92 (1.66) 0.000
50S ribosomal protein L9 AIS07033.1 NO 4.70 (2.06) 0.000 8.17 (3.02) 0.002
30S ribosomal protein S9 AIS07845.1 YES 6.19 (2.19) 0.005 5.86 (1.38) 0.009
50S ribosomal protein L17 AIS07895.1 YES 15.14 (7.44) 0.025 9.64 (4.81) 0.003
50S ribosomal protein L15 AIS07902.1 NO 5.06 (1.54) 0.000 5.92 (1.17) 0.000
50S ribosomal protein L6 AIS07906.1 YES 5.35 (2.12) 0.000 7.59 (1.58) 0.037
30S ribosomal protein S8 AIS07907.1 YES 7.52 (1.56) 0.007 10.76 (2.98) 0.011
50S ribosomal protein L5 AIS07909.1 NO 7.18 (2.38) 0.000 7.73 (2.20) 0.000
50S ribosomal protein L14 AIS07911.1 NO 5.20 (1.87) 0.002 11.07 (12.57) 0.002
50S ribosomal protein L22 AIS07916.1 NO 2.03 (0.76) 0.012 1.89 (0.85) 0.035
50S ribosomal protein L23 AIS07919.1 YES 1.77 (0.58) 0.043 4.45 (1.04) 0.018
  1. The protein profiles produced by A. baumannii grown in modified BALF and in the presence of macrophages were performed using iTRAQ reagents and LC-MS/MS. Differential expression was defined by a relative abundance ratio >1.5 and <0.5
  2. aSecretion prediction are based on (SignalP 4.1 (http://www.cbs.dtu.dk/services/SignalP/), Phobius (http://phobius.sbc.su.se/), PrediSi (http://www.predisi.de/), TatP 1.0 (www.cbs.dtu.dk/services/TatP-1.0), Tatfind 1.4 (http://signalfind.org/tatfind.html), SecretomeP 2.0 (www.cbs.dtu.dk/services/SecretomeP), TMHMM (http://www.cbs.dtu.dk/services/TMHMM/), DAS-TMfilter (http://www.enzim.hu/DAS/DAS.html), LipoP 1.0 (www.cbs.dtu.dk/services/LipoP), DOLOP (http://www.mrc-lmb.cam.ac.uk/genomes/dolop/), and LIPO (http://services.cbu.uib.no/tools/lipo))
  3. bAverage relative protein expression level ratio in sample and control, with the standard deviation in parentheses, quantified by Protein Pilot 4.0 software (ABSciex).
  4. cDetermined by Student’s t test. Values of less than 0.05 are considered significant