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Table 5 RACE-PCR and RT-PRC primers for the four putative seminal fluid proteins and the amplicon sizes

From: Identification and characterisation of putative seminal fluid proteins from male reproductive tissue EST libraries in tiger beetles

  Primers sequence Amplicon size
AcpC01 RACE_F1 5′-GTATTCCATTGTGTCCACCACCTCCGG-3′ 128
RACE_R1 5′-TGGTGGACAAGGTGGACAACATGGAAC -3′
RT_F1 5′-TTGCCCTCCATGTGCAGTAC-3′ 139
RT_R1 5′-TGGCTTCTGTGGCTCAAATTT-3′
AcpC02 RACE_F2 5′- TGAGGAACCAGCCGCACAAGTAAAGAC-3′ 191
RACE_R2 5′-AGACCGACTCTGCAGTTTTTGTCTCGG-3′
RT_F2 5′-AGGAACCAGCCGCACAAGTA-3′ 110
RT_R2 5′-CTCCTTGTTGGGTGGTGCAT-3′
AcpC03 RACE_F3 5′-TCATAACGATGATTCTGCCGCTCGTGG-3′ 176
RACE_R3 5′-GACACTCGAGATGCCTACAGTCCGGTA-3′
RT_F3 5′-ATGCTGTGCTGCTTGTGCAT-3′ 100
RT_R3 5′-GGACAACAGGCCGGAAATG-3′
AcpC04 RACE_F4 5′-ACCAGTTTGTGATTGTCCGCCGTTACG-3′ 300
RACE_R4 5′-GTGTAACTGAACGCACGGGAAATAGCC-3′
RT_F4 5′-GCTATTTCCCGTGCGTTCAG-3′ 100
RT_R4 5′-CGGAGATCTCGTCTGCGTTT-3′