Characterization of a novel chicken muscle disorder through differential gene expression and pathway analysis using RNA-sequencing

Table 1 Top ten upregulated and downregulated genes with fold change for Wooden Breast myopathy-affected birds

Gene	Description	RNA-seq fold change	Nanostring fold change
ENSGAL00000009603	Prolactin-like protein precursor	43.9	N/A
CA3	Carbonic anhydrase III	28.5	25.5
CLEC3A	C-type lectin domain family 3, A	25.3	N/A
KPNA7	Karyopherin alpha 7	24.4	8.0
CSRP3	Cysteine and glycine-rich protein 3	23.2	27.3
CRISP1	Cysteine-rich secretory protein 1	21.5	N/A
FAM132A	Family sequence similarity 132, A	18.0	N/A
MB	Myoglobin	14.3	12.4
CRH	Corticotropin releasing hormone	14.3	13.3
PHGDH	Phosphoglycerate dehydrogenase	11.6	5.4
GC	Group Specific Component	−48.0	N/A
PIT54	PIT 54 protein precursor	−46.0	N/A
FGA	Fibrinogen alpha chain	−20.8	−3.0
FGG	Fibrinogen gamma chain	−18.1	−2.8
OGCHI	Orosomucoid 1	−16.9	N/A
ALB	Albumin	−16.8	−4.0
ENSGAL00000016859	Uncharacterized protein	−14.9	N/A
FGB	Fibrinogen beta chain	−13.3	−3.8
APOH	Apolipoprotein H	−13.1	N/A
YFV	MHC I antigen precursor	−12.9	N/A

This table provides a summary of both the top ten upregulated and downregulated genes found among affected chickens within the sample population. Positive and negative fold change values support, respectively, upregulation and downregulation of gene expression in affected birds relative to the gene expression in unaffected birds. The fold change for each gene is also presented along with the fold change for each gene that was tested using the NanoString nCounter® Technology to verify our expression data

ISSN: 1471-2164