Skip to main content

Table 3 Biocontrol strains and their mutant derivatives that were used in this study

From: Unearthing the genomes of plant-beneficial Pseudomonas model strains WCS358, WCS374 and WCS417

Strain

Relevant characteristics

References

Pseudomonas capeferrum WCS358r

Spontaneous rifampicin-resistant mutant of WCS358. Wild type isolated from potato rhizosphere. Produces siderophore PVD3581

[20]

WCS358-PVD−

Tn5 mutant of WCS358r that does not produce PVD358; Original strain name WCS358-JM213.

[74]

Pseudomonas defensor WCS374r

Spontaneous rifampicin-resistant mutant of WCS374. Wild type isolated from potato rhizosphere. Produces siderophores PVD374 and PSM3742, and the PSM precursor SA3

[20]

WCS374- PVD−

PVD−, PSM+, SA+, Tn5 transposon mutant of WCS374r that does not produce PVD374. Original strain name WCS374-02.

[156]

WCS374- PMS−

PVD+, PSM−, SA+, pmsA mutant4 of WCS374r obtained by site-directed mutagenesis. Original strain name WCS374-4A1.

[24, 70]

WCS374- PVD− PSM−

PVD−, PSM−, SA+, Tn5 transposon mutant of WCS374-4A1 that produces neither PVD374 nor Pseudomonine. Original strain name WCS374-AT12.

[24, 70]

WCS374-PSM− SA−

PVD+, PSM−, SA−, pmsB mutant5 of WCS374r obtained by site-directed mutagenesis. Original strain name WCS374-4B1.

[24, 70]

WCS374-PVD− PSM−-SA−

PVD−, PSM−, SA−, Tn5 transposon mutant of WCS374-4B1 that does not produce PVD374. Original strain name WCS374-BT1.

[24, 70]

Pseudomonas simiae WCS417r

Spontaneous rifampicin-resistant mutant of WCS417. Wild type was isolated from wheat rhizosphere. Produces siderophore PVD417

[21, 48]

WCS417-PVD−

Tn5 mutant of WCS417 that does not produce PVD417. Original strain name WCS417-M634.

[75]

Pseudomonas capeferrum CFBP 2461

Wild-type isolated from bean rhizosphere; produces PVD358

[67]

P. protegens Pf-5

Wild-type isolated from cotton rhizosphere; produces PVD-Pf-5 and enantio-pyochelin

[157]

LK032

Mutant derivative of PF-5 ΔpchA 6ΔpvdI 7 . Does not produce siderophores.

[32]

LK148

Mutant derivative of LK032 ΔpchAΔpvdIΔfpvY. Does not produce siderophores and defective in ferric-pyverdine receptor FpvY

[32]

LK150

Mutant derivative of LK032 ΔpchAΔpvdIΔfpvX. Does not produce siderophores and defective in ferric-pyverdine receptor FpvX

[32]

LK151

Mutant derivative of LK032 ΔpchAΔpvdIΔfpvV. Does not produce siderophores and defective in ferric-pyverdine receptor FpvV

[32]

LK153

Mutant derivative of LK032 ΔpchAΔpvdIΔfpvW. Does not produce siderophores and defective in ferric-pyverdine receptor FpvW

[32]

LK154

Mutant derivative of LK032 ΔpchAΔpvdIΔfpvU . Does not produce siderophores and defective in ferric-pyverdine receptor FpvU

[32]

LK155

Mutant derivative of LK032 ΔpchAΔpvdIΔfpvZ. Does not produce siderophores and defective in ferric-pyverdine receptor FpvZ

[32]

  1. 1PVD, siderophore pyoverdine
  2. 2PSM, siderophore pseudomomine
  3. 3SA, salicylic acid, precursor of pseudomonine and pyochelin
  4. 4 pmsA, gene involved in the synthesis of the histamine moiety of Pseudomonine
  5. 5 pmsB, gene involved in the biosynthesis of SA as a precursor of Pseudomonine
  6. 6 pchA gene encoding an isochorismate synthetase involved in production of SA/pyochelin
  7. 7 pvdI geneencoding a non-ribosomal peptide synthase involved in production of pyoverdine