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Fig. 1 | BMC Genomics

Fig. 1

From: Whole genome capture of vector-borne pathogens from mixed DNA samples: a case study of Borrelia burgdorferi

Fig. 1

Comparison of the mean coverage for B. burgdorferi genomes across capture reactions. a The linear chromosome (910,724 bp) and b the linear plasmid, lp54 (53,657 bp). The y-axis indicates the coverage depth averaged over all samples in each capture reaction, defined as the total number of sequenced bases which map to each nucleotide in the reference B31 genome calculated on a window size of 1000 bp after removal of potential PCR duplicates and reads with mapping quality ≤20. The x-axis indicates the reference position relative to the samples; the light blue region indicates the area within one standard deviation of the mean coverage of samples captured in the capture reaction. GC content (percentage of nucleotide in the genome that are G or C) for the chromosome and the linear plasmid calculated on a 1000 bp window size are shown below the coverage plots

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