Fig. 1

GBP5 is differentially expressed as a function of the PRRS host response QTL across timepoints. Guanylate binding protein 5 (GBP5) is differentially expressed and exhibits allele specific expression (ASE) as a function of rs80800372 genotype at 0,4,7,11 and 14 days post infection (dpi) with Porcine Reproductive and Respiratory Syndrome (PRRS) in PHGC trial 3 RNA-seq data. a Differences in the mean, model normalized gene expression value and log2 fold change values for expression of GBP5 between the AB (blue) and AA (red) genotypes at the SSC4 QTL. *Indicates false discovery rate < 0.05. b Schematic of the GBP5 transcript coding DNA sequence. Variants tested for ASE are shown in black text. The putative causal mutation is shown in red text. The three protein domains shown were identified from the human GBP5 protein as annotated at Ensembl in release 77. The box shown in orange corresponds to the p-loop containing nucleoside triphosphate hydrolase domain (7–279 amino acids (AA)). The box shown in green corresponds to the GBP N and C terminal domains (18–280 AA and 282–577 AA, respectively). The box shown in red corresponds to the CAAX box localization signal required for proper cellular localization of the GBP5 protein. ^{^}The rs340943904 variant is not included in the transcript and is shown here only to provide context since it is predicted to cause a shift in the transcript reading frame and an early stop codon that truncates the 88 C-terminal AA of the protein. c Identification of ASE at 0,4,7,11 and 14 dpi for GBP5 for SNPs in phase with the B rs80800372 allele. The 95 % confidence intervals for estimates of allele proportions are provided