Fig. 3

Validation of differential expression, splicing and allele specific expression of guanylate binding protein 5 (GBP5). GBP5 transcript levels and allelic abundances were analyzed at 0,4,7,10 and 14 days post infection (dpi) with Porcine Reproductive and Respiratory Syndrome (PRRS) virus in PHGC trial 5 RNA-seq data. a Validation of differential expression of GBP5 between the AB (blue) and AA (red) rs80800372 genotypes. *Indicates false discovery rate < 0.05. b Validation of allele specific expression of GBP5 SNPs in phase with the B rs80800372 allele. c Validation of GBP5 differential splicing due to rs80800372 genotype across dpi. Spliced reads uniquely mapping to the three alternate transcripts were analyzed as the percentage of total reads from the RNA-seq data. Both the wild-type and five nucleotide insertion (+5 bps insertion) alleles were differentially spliced between the AA (red bars) and AB (blue bars) rs80800372 genotypes (p < 0.0001; Bonferonni adjusted p < 0.001). There was a trend towards a small difference in the quantity of the retained intron due to rs80800372 genotype (p = 0.06; Bonferonni adjusted p > 0.10). Alternate GBP5 transcripts are labeled on the X-axis