Fig. 7

Expression quantification of miRNA precursors and their targets involved in the weighted correlation networks. Relative quantification by qRT-PCR of miRNA precursors and their corresponding targets. a Expression of miRNAs and corresponding targets in nodules compared to root tissues from the Fig. 6A network. Histograms represent the Log2 fold change level and error bars represent the standard error of the mean of the three expression ratios from three biological replicates. b Expression of novel miRNA precursors from Fig. 6B network. Histograms represent the relative expression and error bars represent the standard error of the mean of the three relative expressions from three biological replicates. Expression values have been standardized at 1 for the control roots and proportionally adjusted for nodule expression. The geometric mean of the expression of three housekeeping genes was used for normalization of the expression levels