Fig. 7

Western blot analysis and validation of several key proteins from microarray results. A549 cells were collected and lysed after 1-min NTP treatment at different time points, namely, 2, 4, 8, 16 and 24 h. With SDS-PAGE gel electrophoresis, the expressions of Caspase3, PARP, mTOR, phospho-mTOR, Akt, phospho-Akt, NF-κB, phospho-IκBα, JNK, phospho-JNK, p38 MAPK, phospho-p38 MAPK, c-Jun, JunB, c-Fos, and ACTB were measured.