Comparative proteomic analysis of drought tolerance in the two contrasting Tibetan wild genotypes and cultivated genotype

Wang, Nanbo; Zhao, Jing; He, Xiaoyan; Sun, Hongyan; Zhang, Guoping; Wu, Feibo

doi:10.1186/s12864-015-1657-3

Table 2 Proteins whose expression was significantly induced (+) in XZ5 leaves but down-regulated (-) /unchanged in XZ54, or unchanged in XZ5 but down-regulated in XZ54 after 2 and 5 days of recovery following drought stress

From: Comparative proteomic analysis of drought tolerance in the two contrasting Tibetan wild genotypes and cultivated genotype

Spot No.	Protein name	C. I. (%)	Accession number	MW (Da)	pI	AASC (%)	Fold increase (+) or decrease (-)			Function
Spot No.	Protein name	C. I. (%)	Accession number	MW (Da)	pI	AASC (%)	XZ5	XZ54	ZAU3	Function
	Two days of recovery
C1	Ribulose bisphosphate carboxylase/oxygenase activase, chloroplast precursor [Hordeum vulgare]	100	Q40073\|RCAA_HORU	51041	8.0	41.8	+1.6	−1.7	−1.9	Photosynthesis
C2	Melanoma-associated antigen p97 [G. gallus]	96.2	gi\|45383930	80860	5.9	22.0	+10⁶	NE	NE	Stress response
(A3)^a	Melanoma-associated antigen p97 [G. gallus]	96.2	gi\|45383930	80860	5.9	22.0	+10⁶	NE	NE	Stress response
C3	Type I chlorophyll a/b-binding protein b [A. tricolor]	100	gi\|13676406	16608	4.6	18.8	+10⁶	NE	NE	Photosynthesis
(A4 B1)	Type I chlorophyll a/b-binding protein b [A. tricolor]	100	gi\|13676406	16608	4.6	18.8	+10⁶	NE	NE	Photosynthesis
C4	Glyceraldehyde-3-phosphate dehydrogenase 2 [A. thaliana]	100	gi\|186478427	37644	7.6	14.6	+3.3	−1.2	−10⁶	Metabolism
C5	Os02g0739600 Putative pyruvate dehydrogenase E1 alpha subunit [Oryza sativa]	100	gi\|115448577	42675	7.6	34.6	+3.0	−1.3	−1.4	Metabolism
C6	Elongation factor G [A. thaliana]	100	gi\|62320532	47645	5.4	21.4	+1.2	−1.7	−1.7	Amino-acid biosynthesis
C7	Elongation factor G [A. thaliana]	100	gi\|62320532	47645	5.4	21.4	+1.2	−1.7	−1.7	Amino-acid biosynthesis
C8	Putative ascorbate peroxidase [T. aestivum]	100	gi\|25992559	39922	5.5	5.5	+5.5	+1.2	−1.9	Stress response
C9	FTSH1 (FtsH protease 1); ATP-dependent peptidase/ATPase/ metallopeptidase [A. thaliana]	100	gi\|18402995	76712	5.6	19.0	−0.02	−1.7	−3.9	Stress response
(A11 D4)		100	gi\|18402995	76712	5.6	19.0	−0.02	−1.7	−3.9	Stress response
C13	Thioredoxin M-type, chloroplast precursor (Trx-M)	100.0	gi\|11135474	19120	8.7	44.0	+1.9	−1.6	−1.1	Stress response
	Five days of recovery
D1	Hypothetical protein [Sporobolus stapfianus]	99.9	gi\|1808684	31866	5.7	21.8	+10⁶	+1.3	+1.4	Unknown
D2	Os05g0405000 [O. sativa]	100	gi\|115463815	102722	6.0	18.5	+1.8	−2.7	−5.7	Photosynthesis
D3	Ribulosebisphosphate carboxylase large chain precursor [P. sativum]	98.8	P04717\|RBL_PEA	52730	6.6	25.5	−0.03	−1.5	−2.2	Photosynthesis
(A10)		98.8	P04717\|RBL_PEA	52730	6.6	25.5	−0.03	−1.5	−2.2	Photosynthesis
D4	FTSH1 (FtsH protease 1); ATP-dependent peptidase/ATPase/ metallopeptidase [A. thaliana]	100	gi\|18402995	76712	5.6	19.0	−1.4	−2.3	−6.3	Stress response
(A11 C9)		100	gi\|18402995	76712	5.6	19.0	−1.4	−2.3	−6.3	Stress response
D5	ATP synthase CF1 beta subunit [H. vulgare]	100	gi\|118430395	53841	5.2	52.8	+2.6	−0.08	+1.5	Energy

AASC, Amino acid sequence coverage; Protein spot ID refers to numbers in Fig. 1c-d. Accession number of top database match from the NCBInr database. Fold increase and decrease were calculated as drought/control, and –control/drought for up and down -regulated proteins respectively. All ratios shown are statistically significant (p < 0.05). +10000 and -10000 referred to the specific expressed and totally inhibited proteins, respectively. NE, non expression. ^a the same protein spots identified at different stages

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ISSN: 1471-2164

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