Fig. 4

RT-qPCR validation of genes under apparent inverse regulation by CjFur/CjPerR under iron limitation. RT-qPCR of selected genes in the Δfur (Panel a), ΔperR (Panel b) and ΔfurΔperR (Panel c) strains under iron limitation. Genes colored in blue show reduced expression in the Δfur mutant, increased expression in the ΔperR mutant and no change in expression in the ΔfurΔperR mutant in both the RNA-seq data and the RT-qPCR validation. Genes in green are differentially expressed in the ΔperR/ΔfurΔperR mutants via RT-qPCR but only the ΔperR under RNA-seq. Cj1386 was found to be differentially expressed in the ΔperR/ΔfurΔperR mutants under both techniques. AhpC and chuA are negative controls for metal dependant CjFur/CjPerR regulation. All expression values are relative to wild-type and normalized to slyD. Asterisks denote fold changes >1.5 with a p value <0.05