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Fig. 1 | BMC Genomics

Fig. 1

From: piRNA-like small RNAs mark extended 3’UTRs present in germ and somatic cells

Fig. 1

Deep sequencing of adult testis small RNAs does not capture the full complexity of piRNA-like species. Even at extreme read depths, many new unique alignments are identified. a The number of observed unique sequences increases as a power function of read depth in our dataset (black line: experimental data; grey line: logarithmic fit). The curve does not approach saturation even at read depth >1.2x108. b Frequency with which sequences are observed in the dataset. Most are observed only once or a few times. c Length distribution of uniquely aligned small RNAs in adult testis, with duplicate reads removed. There is a distinct peak at 29 nt, with a distribution skewed toward smaller sizes. This size distribution is typical of adult testis piRNAs. d Genomic annotation of uniquely aligned small RNAs in testis. The concentration in intergenic regions is typical of piRNAs from the adult testis. Repeat: 20 %; mRNA: 12 %; intron: 5 %; intergenic: 63 %

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