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Fig. 4 | BMC Genomics

Fig. 4

From: Co-cultivation and transcriptome sequencing of two co-existing fish pathogens Moritella viscosa and Aliivibrio wodanis

Fig. 4

Growth and inhibition zones from bacterial cross streaks and on soft agar overlays. Representative A. wodanis strains positive for the bacteriocin locus (Aw+) were vertical streaked onto blood agar plates: A) A. wodanis 88/09/441, B) A. wodanis 06/09/139. Prependicular and in horizontal streaks: Mv1, M. viscosa 3632; Mv2, M. viscosa 06/09/139; Aw1+ (positive for the bacteriocin locus), A. wodanis 90/09/325; Aw2, A. wodanis 02/09/569. Reduced growth in the vicinity of Aw+ are illustrated for both M. viscosa (a) with Mv2 and for A. wodanis (b) with Aw2 where a clearly defined inhibition zone is observed. In the agar overlay method (c) and (D Aw1+ and Aw2 were first spotted onto the plates and grown to smal colonies before the agar overlay was added and bacterial suspensions of Mv2 (C) and Aw1+ (d) were plated on top. Colonies (Aw1+) surrounded by inhibition zones (marked with *) in the on-top bacterial lawn indicate bacteriocin activity

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BMC Genomics

ISSN: 1471-2164

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