Fig. 1

Percent of genes with the majority of reads mapping within each gene quartile. All identified protein coding genes were divided into four even quartiles numbered from 5′ - > 3′ position relative to the gene sequence. The number of reads whose 5′ most base resided in a given quartile (x-axis) was counted, and the percent of genes (y-axis) with the majority of reads in a given quartile was plotted. The total number of genes analyzed was: 25842, 21804, 20552, 20909, 20567, 19095, 20438, 19432, 19733, and 20785 for samples S1BALA, S2BPLP, S3BPLP, S4BPLP, S5BPLA, S6BPLA, S7BPLA, S8BPLA, S9BPLA, and S10BPLA respectively, with 22468 and 22223 genes analyzed for samples in experiment 2 and 3 combined, respectively. Bracketed bars with a ‘*’ symbol denotes libraries prepared with a linker sequence in the template switching oligo