Gene expression analysis of skin grafts and cultured keratinocytes using synthetic RNA normalization reveals insights into differentiation and growth control

Table 1 Ten most correlated genes with PC1. Tables a and b are subsets of significantly correlated genes with relevant functions in skin lineage (for full data, see Additional file 9: Table S6). a Positively correlated genes, which were upregulated in SGs, and (b) negatively correlated genes, which were upregulated in KCs and HaCaTs

Gene Symbol	Score	Local FDR
(a)
RAB11FIP1	0.934	0.00044
KRT80	0.933	0.00045	Localization around desmosomal plaques in earlier stages of differentiation [PMID:20843789]
ID4	0.915	0.00052	Lack of the protein in parakeratotic cells at upper skin layer [PMID:21663940]
PPL	0.911	0.00053	A component of desmosomes and of the cornified envelope [PMID:9412476]
KRT1	0.911	0.00054	Specifically expressed in the spinous and granular layers [PMID:10511477]
BCL6	0.900	0.00058	Expression at the terminal differentiation stage [PMID:8912662]
ERBB3	0.897	0.00059	Skin biopsy expressed more than the cultured cells [PMID:11571634]
PLAC2	0.896	0.00059	lncRNA controlling terminal differentiation [PMID:23201690]
PKP1	0.896	0.00059	Localization around desmosomal plaques and nuclei [PMID:16632867]
KRT10	0.894	0.00060	Specifically expressed in the spinous and granular layers [PMID:10511477]
(b)
PRDX3	−0.922	0.00044	Mitochondrial [PMID:17893648]
SEC61G	−0.914	0.00045
NDUFB3	−0.914	0.00052	Mitochondrial [PMID:12611891]
FXC1	−0.913	0.00053	Mitochondrial [PMID:14726512]
ATP5J	−0.911	0.00054	Mitochondrial [PMID:12110673]
GNG10	−0.911	0.00058
TXNDC9	−0.911	0.00059
MRPS23	−0.910	0.00059	Mitochondrial ribosomal protein
ENY2	−0.910	0.00059
PSMB2	−0.909	0.00060

ISSN: 1471-2164