Fig. 1

TS score explanation. a Hypothetical examples of time course transcript expression profiles from a gene to illustrate the concept of the TS score. The primary transcript is shown in blue. Minor transcripts are shown in other colours. The red line would have a high TS score since it has reasonably high expression relative to the primary transcript but with a different shape. The green transcript would have a Low TS score since although it has high general expression it has the same shape as the primary transcript. The purple transcript would have a low TS score since although it has a different shape it has low expression relative to the primary transcript. b, Example of a secondary isoform taken from the ‘sdt’ gene with a high TS score when compared to the primary isoform. The legend IDs between brackets are FlyBase transcript ID’s. Along the x-axis the first 24 hours correspond to embryogenesis stages; time points labelled ‘L’ correspond to subsequent Larval developmental stages; WPP indicates the white prepupae and,‘P’ indicates pupal stage, ‘F’ and ‘M’ are for female and male adult stages respectively (see Additional file 1: Figure S1 for an example of a gene with a low TS score)