Fig. 3

Stability of transcripts from spike-ins and indigenous targets in stool mRNA for up to 15 days. Absolute copy numbers of the spikes sFGFP (a) and mCherry (b) and of the two indigenous targets 23S rRNA gene (c) and GAPDH gene (d) as determined by quantitative RT-PCR in stool samples. Samples were stored in RNA Later at room temperature (red graph) or at 4 °C (blue graph) or in RNA Protect at RT (black graph)