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Table 3 Analysis of gene loss in X and Y chromosomes combining BAC and RNA-seq data

From: Identifying new sex-linked genes through BAC sequencing in the dioecious plant Silene latifolia

Categories of genes

X-linked genes

Y-linked genes

All new genes in BAC sequences

49

10

No match to RNA-seq contigs

6

3

Genes retained for analysis

43

7

Category (i): X/Y gene pair results in RNA-seq analysis

16

2

Category (ii): X-hemizygous results in RNA-seq analysis

5

1

Category (iii): Not ascertained as sex-linked by RNA-seq analysis

22

4

Estimated X/Y false negative rate for gene pairs for RNA-seq analysisa

25 %

25 %

Expected number of XY pairs undetected in RNA-seq analysis

10.75

1.75

Potential number of X-hemizygous (X0) or Y0 genes undetected in RNA-seq analysis

11.25

2.25

Potential total number of X-hemizygous (X0) or Y0 genes (sum of detected + undetected in RNA-seq analysis numbers above)

16.25

2.25

Potential proportion of X-hemizygous (X0) or Y0 genesb

27-33 %

4-5 %

  1. aBased on 39 genes previously known to have X-linked and Y-linked copies, see Additional file 4: Table S4
  2. bBased on total numbers of potential ancestral genes, either including the probe genes, or excluding them, respectively (see text for details).