Fig. 4

Read count cluster statistics and genotype quality scores for the 60-loci libraries. a: Mean versus standard deviation of clusters obtained with the E libraries. Black and blue circles indicate, respectively, low and high read count clusters. Despite locus-to-locus variations, most clusters had similar means and standard deviations. We manually set thresholds (represented as gray lines) at 3 (mean) and 0.5 (standard deviation), which dropped out locus 23 (low read count cluster had mean greater than 3), loci 50 and 57 (high read count cluster had mean less than 3) and locus 30 (standard deviation greater than 0.5). b: Same as a for the G libraries. We dropped locus 34 (high read count cluster had mean less than 3) and loci 3, 43 and 44 (standard deviation greater than 0.5). c: Histograms of genotype quality scores obtained for the E libraries. Scores below 7 (threshold indicated by a gray vertical line) are indicated as crosses in Fig. 3a, c. d: Same as c for the G libraries