Fig. 2

a qRT-PCR analysis of the pkn22 transcripts in absence (white bars) or presence of 100 μM H₂O₂ during 1 h (grey bars). Data are shown as fold-change between normal and stress conditions. Each sample was measured in triplicate and the standard deviation is indicated by error bars. Values were normalized to the rnpB transcript. The value obtained for the condition minus H₂O₂ was set to 1. RNAs were extracted from Nostoc wild type strain (WT) or from a recombinant strain expressing the furA gene from the petE promoter (WT/petE-furA). b qRT-PCR analysis of the pkn22 transcripts in absence (white bars) or presence (grey bars) of 100 μM H₂O₂ during 1 h. Data are expressed as fold-change from normal conditions. Each sample was measured in triplicate and the standard deviation is indicated by error bars. Values were normalized to the rnpB transcript. The value obtained for the condition minus H₂O₂ was set to 1. RNAs were extracted from Nostoc wild type strain (WT) or from the ntcA mutant (CSE2). c Quantitative RT-PCR analysis of the pkn22 transcripts at different times (3–8 and 24 h) after nitrogen step down. RNAs were extracted from the wild type strain () or the CSE2 strain (). Data are expressed as fold-change from normal conditions. Each sample was measured in triplicate and the standard deviation is indicated by error bars. Values were normalized to the rnpB transcript