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Table 1 Validation of the microarray data by quantitative RT-PCR approach for selected representative genes. Fold change values are in Log2

From: The Pkn22 Ser/Thr kinase in Nostoc PCC 7120: role of FurA and NtcA regulators and transcript profiling under nitrogen starvation and oxidative stress

Gene

Microarray + H2O2/-H2O2 fold change

QRT-PCR + H2O2/-H2O2 fold change

Wild type

pkn mutant

pknC strain

Wild type

pkn mutant

pknC strain

Alr4616

0.282 ± 0.36

2.321 ± 0.36

0.375 ± 0.36

0.433 ± 0.36

3.65 ± 0.41

0.136 ± 0.36

All4791

0.304 ± 0.11

2.040 ± 0.36

0.405 ± 0.36

0.398 ± 0.36

2.33 ± 0.22

0.213 ± 0.36

All2724

0.037 ± 0.41

2.428 ± 0.28

−0.151 ± 0.36

0.124 ± 0.36

2.56 ± 0.0.37

−0.104 ± 0.11

Alr2879

0.541 ± 0.09

−1.871 ± 0.36

−0.331 ± 0.36

−0.446 ± 0.4

−2.024 ± 0.18

−0.254 ± 0.36

All4780

−0.680 ± 0.22

−2.437 ± 0.39

−0.902 ± 0.36

−0.789 ± 0.36

−3.012 ± 0.54

−0.489 ± 0.36

All0167

−0.661 ± 0.40

−1.784 ± 0.36

0.389 ± 0.36

−0.573 ± 0.36

−1.856 ± 0.39

0.127 ± 0.36

Gene

Microarray -N/+N fold change

QRT-PCR -N/+N fold change

Wild type

pkn mutant

pknC strain

Wild type

pkn mutant

pknC strain

Alr0970

−3.964 ± 0.20

1.939 ± 0.71

−2.399 ± 0,33

−2.521 ± 0,17

2.001 ± 0,22

−2.207 ± 0,19

Alr0657

−0.960 ± 0,31

2.764 ± 0,16

−1.013 ± 0,58

−0.677 ± 0,08

2.814 ± 0,36

−0.899 ± 0,15

Alr3303

0.279 ± 0,14

3.064 ± 0,22

0.420 ± 0,19

0.311 ± 0,61

2.996 ± 0,07

0.336 ± 0,29

Alr0020

−0.365 ± 0,50

−1.915 ± 0,81

−0.255 ± 0,21

−0.432 ± 0,78

−2.428 ± 0,18

−0.342 ± 0,48

All1651

0.904 ± 0,09

−2.376 ± 0,31

0.710 ± 0,39

0.872 ± 0,29

−3.010 ± 0,60

0.621 ± 0,55

All3181

2.980 ± 0,11

−1.807 ± 0,19

1.978 ± 0,46

3.017 ± 0,46

−1.995 ± 0,09

2.406 ± 0,67