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Table 3 Measurement of intracellular iron content. Nostoc wild type strain was grown in presence of nitrate (BG11) or in combined nitrogen depleted medium (BG11o), or exposed to peroxide stress after nitrogen step down (BG11o H2O2) (see Experimental procedures), before the measurements were carried out. The results are the means of three independent experiments. The standard deviations are indicated

From: The Pkn22 Ser/Thr kinase in Nostoc PCC 7120: role of FurA and NtcA regulators and transcript profiling under nitrogen starvation and oxidative stress

Strain

Growth conditions

Iron content (μg/g of dried cells)

Wild type

BG11

159.13

±15.26

BG11o

1970.02

±176.74

BG11o + H2O2

1507.42

±190.88

WT#pkn22

BG11

112.69

±23.04

BG11o

1246.31

±101.42

BG11o + H2O2

1462.57

±165.64

WT#pkn22/petE-pkn22

BG11

123.89

±19.84

BG11o

1567.24

±123.05

BG11o + H2O2

1987.02

±167.32