Fig. 1

Comparison of the stress sensitivities of control and ΔatfA Aspergillus nidulans strains. Plates were point-inoculated with freshly grown conidia (10⁵ conidia in 5 μl aliquots of 0.9 % NaCl, 0.01 % Tween 80) and were incubated at 37 °C for 5 d (Yin et al., 2013 [63]). All assays were carried out in triplicates, and representative photos are presented here. The stress sensitivites of the ΔatfA strain were always higher than those of the control at any concentrations of the oyidative stress generating agents tested. In contrast, there was no difference between the relative growths of the mutant and control strains when exposed to NaCl. The employed stressor concentrations were: tBOOH: 0.8 mM, H₂O₂: 6.0 mM, MSB: 0.12 mM, diamide: 2.0 mM and NaCl: 0.6 M. Representative photos are presented