Fig. 8
From: Systematically dissecting the global mechanism of miRNA functions in mouse pluripotent stem cells
DNA methylation mediates miRNA activations in stem cells. a, The methylation levels upstream and downstream from the miRNA start site. Down-regulated miRNAs have significantly higher methylation in upstream region when compared with up-regulated miRNAs. b, Detailed methylation profiling for regions that are 2000Â bp upstream from the activated miRNAs. The top 30 down-regulated miRNAs (Additional file 1: Table S2) have higher methylation around -1000Â bp (green highlighted in middle panel) than that of the top 30 up-regulated miRNAs (Additional file 1: Table S2, upper panel). The methylation profiling of a single down-regulated miRNA-133 as a representative example (bottom panel). c. Negative correlation between DNA methylation and miRNA expression. Red line represents regression line