Genes involved in sex pheromone biosynthesis of Ephestia cautella, an important food storage pest, are determined by transcriptome sequencing

Antony, Binu; Soffan, Alan; Jakše, Jernej; Alfaifi, Sulieman; Sutanto, Koko D.; Aldosari, Saleh A.; Aldawood, Abdulrahman S.; Pain, Arnab

doi:10.1186/s12864-015-1710-2

Table 3 Putative pheromone biosynthesis enzymes (PBEs) in the E. cautella PG

From: Genes involved in sex pheromone biosynthesis of Ephestia cautella, an important food storage pest, are determined by transcriptome sequencing

Unigene	Accession no.	Length (bp)	Putative identification	Species	Accession no.	Blast Hit score	E-value	% of identity	RPKM
PBAN receptor
EP_Contig_27375_PBAN	GBXH01027379	3094	PBAN receptor isoform C	Ostrinia nubilalis	AGL12068	479	1.00E-155	63.1	4.39
EP_Contig_24961_PBAN	GBXH01024977	482	PBAN receptor isoform A	Ostrinia nubilalis	AGL12066	103	4.00E-23	60.1	0.86
Fatty acid Transport Protein
EP_Unigene_1_FATP	GBXH01082863	2057	long chain fatty acid transport protein 1	Bombyx mori	XP_004927673	675	0	67.6	46.52
EP_Unigene_2_FATP	GBXH01082864	1917	long chain fatty acid transport protein 4	Nasonia vitripennis	XP_001603871	668	0	73.8	100
EP_Unigene_3_FATP	GBXH01082865	966	Fatty acid transport protein	Ostrinia scapulalis	BAJ33524	555	0	80.8	140
EP_Contig_1647_FATP	GBXH01001738	366	Fatty acid transport protein	Eilema japonica	BAJ33523	190	3.78E-54	79.9	120
EP_Contig_12202_FATP	GBXH01012261	526	Fatty acid transport protein	Papilio xuthus	BAM19873	157	5.88E-44	81.4	33
Acetyl CoA carboxylase
EP_Unigene_1_ACC	GBXH01000029	1938	Acetyl CoA carboxylase isoform b	Agrotis ipsilon	AGR49308	926	0	83.4	122
EP_Unigene_2_ACC	GBXH01000030	1464	Acetyl CoA carboxylase	Agrotis ipsilon	AGR49308	834	0	84.8	91
EP_Unigene_3_ACC	GBXH01000031	1342	Acetyl CoA carboxylase-like	Bombyx mori	XP_004930758	803	0	93.4	70
EP_Unigene_4_ACC	GBXH01000032	607	Acetyl CoA carboxylase	Danaus plexippus	EHJ73343	394	1.40E-126	89.8	64
EP_Contig_63068_ACC	GBXH01062648	205	Acetyl CoA carboxylase	Agrotis ipsilon	AGR49309	64	5.50E-11	77.5	9
EP_Contig_14940_ACC	GBXH01014992	776	Acetyl CoA carboxylase	Agrotis ipsilon	AGR49308	300	8.70E-89	67.4	74
Fatty acid synthase
EP_Contig_284_FAS	GBXH01000378	3493	fatty acid synthase	Agrotis ipsilon	AGR49310	1254	0	67.5	53
EP_Contig_1101_FAS	GBXH01001195	2074	putative fatty acid synthase	Danaus plexippus	EHJ78836	473	5.22E-143	68.6	109
EP_Contig_8286_FAS	GBXH01008363	1991	fatty acid synthase	Agrotis ipsilon	AGR49310	1127	0	82.3	51
EP_Contig_42681_FAS	GBXH01042586	718	fatty acid synthase-like	Bombyx mori	XP_004927661	73	2.21E-11	51	0.3
EP_Contig_55530_FAS	GBXH01055262	1079	fatty acid synthase-like	Bombyx mori	XP_004922805	228	6.32E-61	55.2	0.7
EP_Contig_69718_FAS	GBXH01069102	665	fatty acid synthase-like	Bombyx mori	XP_004925618	137	9.52E-33	55.8	0.39
EP_Contig_72627_FAS	GBXH01071942	325	fatty acid synthase-like	Bombyx mori	XP_004922805	105	1.68E-23	65.3	0.12
EP_Contig_74719_FAS	GBXH01073973	714	fatty acid synthase-like	Bombyx mori	XP_004925618	179	2.99E-47	54.7	0.33
EP_Contig_74831_FAS	GBXH01074081	260	fatty acid synthase-like	Bombyx mori	XP_004925618	121	2.49E-26	68.2	0.18
EP_Contig_76686_FAS	GBXH01075900	518	fatty acid synthase-like	Bombyx mori	XP_004925618	146	1.19E-36	59.4	0.18
EP_Contig_79616_FAS	GBXH01078764	345	fatty acid synthase-like	Bombyx mori	XP_004922805	72	6.27E-12	59.1	0.12
EP_Contig_81802_FAS	GBXH01080886	342	fatty acid synthase-like	Bombyx mori	XP_004922805	170	4.53E-46	83.6	0.15
Desaturase
EP_Unigene_3_DES	GBXH01000080	868	desaturase-like protein oblr-fb7a	Choristoneura rosaceana	AAN39698	130	5.38E-60	88.2	284
EP_Unigene_4_DES	GBXH01000081	744	terminal desaturase	Amyelois transitella	AGO96562	452	2.30E-157	86.1	3391
EP_Unigene_7_DES	GBXH01000082	1227	desaturase-like protein sfwg-nf-b	Ctenopseustis herana	AER29846	376	1.04E-124	68.8	261
EP_Unigene_9-1286_DES	GBXH01000083	932	terminal desaturase	Amyelois transitella	AGO96562	221	1.14E-135	79.3	4967
EP_Unigene-10_DES	GBXH01000084	875	stearoyl-coa desaturase	Bombyx mori	NP_001274329	357	4.81E-119	66.4	38
EP_Unigene11_14851DES	GBXH01000085	678	acyl- z9 desaturase	Agrotis ipsilon	AGR49313	150	1.64E-65	81	55
EP_Unigene_12_DES	GBXH01000086	636	acyl- delta desaturase	Bombyx mori	XP_004932163	333	7.73E-112	82	0.6
EP_Contig_ 343_DES	GBXH01000437	2168	delta 11 desaturase	Amyelois transitella	AGO96562	288	4.00E-87	70.56	1739
EP_Contig_ 5930_DES	GBXH01006012	1002	Acyl-desaturase	Bombyx mori	XP_004929766	364	7.73E-121	86.7	41
EP_Contig_ 20984_DES	GBXH01021017	874	acyl-delta-9 desaturase	Manduca sexta	CAJ27975	363	3.07E-134	95.6	1.82
EP_Contig_ 25772_DES	GBXH01025783	405	acyl-delta-9-3a-desaturase	Danaus plexippus	EHJ76461	150	6.39E-41	89	19
EP_Contig_ 63178_DES	GBXH01062755	207	Acyl-desaturase	Heliothis virescens	AGO45840	131	1.50E-36	96.3	0.98
EP_Contig_ 69106_DES	GBXH01068508	631	acyl-delta desaturase-like	Bombyx mori	XP_004925564	160	2.74E-43	68.5	0.74
EP_Contig_ 81260_DES	GBXH01080359	330	acyl-z6 desturase	Lampronia capitella	ABX71630	79	2.96E-15	58.9	0.16
EP_Contig_ 37061_DES	GBXH01036958	1551	acyl-delta desaturase-like	Bombyx mori	XP_004925564	206	4.96E-57	76.4	1.02
EP_Contig_ 27034_DES	GBXH01027039	1923	Acyl-desaturase	Spodoptera littoralis	AAQ74260	542	0	83.2	20
EP_Contig_ 36616_DES	GBXH01036561	379	acyl-delta desaturase-like	Bombyx mori	NP_001274329	57	3.07E-07	76	15
EP_Contig_ 70932_DES	GBXH01070286	612	desaturase	Ostrinia nubilalis	ADB25212	163	6.68E-45	70.7	0.32
EP_Contig_ 37918_DES	GBXH01037854	1028	Acyl-desaturase	Spodoptera exigua	AFO38465	399	5.46E-134	89	3
EP_Contig_ 71065_DES	GBXH01070415	724	acyl-detla9-4-desaturase	Dendrolimus punctatus	ABX71813	153	2.12E-40	70.4	0.2
EP_Contig_145_DES	GBXH01000239	330	terminal desaturase	Ctenopseustis obliquana	AER29852	267	2.00E-54	72.4	1752
Fatty Acyl Reductase
EP_Unigene_ 1_FAR	GBXH01082835	1235	fatty-acyl CoA reductase 5	Ostrinia nubilalis	ADI82778	399	8.28E-131	77.3	743
EP_Unigene_ 2_FAR	GBXH01082836	1791	putative fatty acyl-CoA reductase CG8306-like isoform X1	Bombyx mori	XP_004930778	848	0	77	503
EP_Unigene_ 3_FAR	GBXH01082837	767	putative fatty acyl-CoA reductase CG5065-like	Bombyx mori	XP_004926012	117	5.08E-38	62	487
EP_Unigene_ 4_FAR	GBXH01082838	1529	fatty-acyl CoA reductase 1	Ostrinia nubilalis	ADI82774	581	0	77.1	41
EP_Unigene_ 5_FAR	GBXH01082839	1053	fatty-acyl CoA reductase 4	Ostrinia nubilalis	ADI82777	372	7.69E-122	73.1	74
EP_Unigene_ 6_FAR	GBXH01082840	933	fatty-acyl CoA reductase 2	Ostrinia nubilalis	ADI82775	466	3.48E-157	70.2	84
EP_Unigene_ 7_FAR	GBXH01082841	778	putative fatty acyl-CoA reductase CG5065-like	Bombyx mori	XP_004930776	385	5.10E-128	87.9	24
EP_Unigene_ 8_FAR	GBXH01082842	2493	putative fatty acyl-CoA reductase CG5065-like	Bombyx mori	XP_004930522	861	0	80.6	82
EP_Unigene_ 9_FAR	GBXH01082843	2019	fatty-acyl CoA reductase 5	Danaus plexippus	EHJ72233	223	8.62E-69	67.6	772
EP_Unigene_ 10_FAR	GBXH01082844	1280	putative fatty acyl-CoA reductase CG5065-like	Bombyx mori	XP_004925992	736	0	79.7	21
EP_Unigene_ 11_FAR	GBXH01082845	1203	fatty-acyl CoA reductase 6	Ostrinia nubilalis	ADI82779	492	5.84E-168	64.2	30
EP_Unigene_ 12_FAR	GBXH01082846	839	fatty-acyl CoA reductase 4	Ostrinia nubilalis	ADI82777	345	2.17E-112	73.4	65
EP_Unigene_ 14_FAR	GBXH01082847	594	putative fatty acyl-CoA reductase CG5065-like	Danaus plexippus	XP_004926010	108	1.47E-24	59.1	536
EP_Unigene_ 15_FAR	GBXH01082848	565	fatty-acyl CoA reductase 6, partial	Agrotis ipsilon	AGR49321	142	1.12E-37	65	3
EP_Contig_ 2421_FAR	GBXH01002511	433	Fatty-acyl CoA reductase 2	Ostrinia nubilalis	ADI82775	266	1.09E-83	86.71	41
EP_Contig_ 6194_FAR	GBXH01006275	1721	putative fatty acyl-CoA reductase CG5065-like	Bombyx mori	XP_004926017	516	5.33E-174	82.65	25
EP_Contig_ 45618_FAR	GBXH01045493	236	putative fatty acyl-CoA reductase CG5065-like	Bombyx mori	XP_004929542	58	5.32E-08	41.5	1
EP_Contig_ 11410_FAR	GBXH01011473	251	fatty-acyl CoA reductase 2	Ostrinia nubilalis	ADI82775	117	1.63E-28	67.5	30
EP_Contig_ 13590_FAR	GBXH01013646	516	putative fatty acyl-CoA reductase CG5065-like	Bombyx mori	XP_004929542	48	8.71E-09	50	43
EP_Contig_ 65474_FAR	GBXH01064989	483	putative fatty acyl-CoA reductase CG5065-like	Bombyx mori	XP_004925993	165	5.33E-45	75.69	0.5
EP_Contig_ 56254_FAR	GBXH01055966	473	fatty-acyl CoA reductase 5	Danaus plexippus	EHJ72233	177	3.18E-49	52.78	2
EP_Contig_ 10215_FAR	GBXH01010281	3493	putative fatty acyl-CoA reductase CG5065-like	Bombyx mori	XP_004930776	549	1.90E-179	81.94	15
EP_Contig_ 53541_FAR	GBXH01053315	1771	fatty-acyl CoA reductase 4	Ostrinia nubilalis	ADI82777	528	3.49E-179	53.76	0.6
EP_Contig_ 53189_FAR	GBXH01052971	1006	fatty-acyl CoA reductase 5	Danaus plexippus	EHJ72233	422	5.00E-141	63.21	2
EP_Contig_ 61889_FAR	GBXH01061492	326	putative fatty acyl-CoA reductase CG5065-like	Bombyx mori	XP_004929961	146	2.44E-39	79.49	0.24
EP_Contig_ 72742_FAR	GBXH01072052	225	putative fatty acyl-CoA reductase CG5065-like	Bombyx mori	XP_004929542	64	9.35E-10	40	0,16
EP_Contig_ 78653_FAR	GBXH01077818	271	FAR-like protein VI	Ostrinia scapulalis	ACJ06513	156	5.00E-32	58	0.3
EP_Contig_ 79681_FAR	GBXH01078826	384	putative fatty acyl-CoA reductase CG5065-like	Bombyx mori	XP_004925993	205	1.55E-60	81.89	0.25
Fatty acetyltransferase
EP_Unigene_ 2_FAT	GBXH01082849	1054	Acetyltransferase 1 [cl21486]	Danaus plexippus	EHJ65205	371	7.20E-123	83.7	65
EP_Unigene_ 3_FAT	GBXH01082850	949	n-acetyltransferase esco1 [cl16450]	Bombyx mori	XP_004925351	366	1.79E-115	65	5
EP_Unigene_ 4_FAT	GBXH01082851	2303	n-alpha acetyltransferase [cl09317]	Bombyx mori	XP_004932434	648	0	78.1	28
EP_Unigene_ 5_FAT	GBXH01082852	1351	Acetyltransferase 1 [cl09938]	Danaus plexippus	EHJ65205	296	4.25E-92	65.3	2
EP_Unigene_ 6_FAT	GBXH01082853	916	Putative acetyltrasnferase [predicted]	Danaus plexippus	EHJ75659	124	4.28E-30	52.1	1.2
EP_Unigene_ 7_FAT	GBXH01082854	2115	n-acetyltrasnferase [cl17182]	Danaus plexippus	EHJ73917	350	8.18E-113	93.7	45
EP_Unigene_ 8_FAT	GBXH01082855	1577	n-acetyltrasnferase mak-3 like protein [cl17182]	Bombyx mori	XP_004928263	360	1.27E-117	78	43
EP_Unigene_ 9_FAT	GBXH01082856	1527	arylalkylamine n-acetyltrasnferase [cl17182]	Biston betularia	ADF43200	376	3.24E-125	73.9	91
EP_Unigene_ 10_FAT	GBXH01082857	1369	n-alpha-acetyltrasnferase 60-like [cl17182]	Bombyx mori	XP_004931652	471	1.90E-162	77.6	10
EP_Unigene_ 12_FAT	GBXH01082858	496	acetyltrasnfease1 [cl09938]	Ostrinia scapulalis	BAH03386	205	8.39E-61	90.2	112
EP_Unigene_ 13_FAT	GBXH01082859	495	n-alpha-acetyltransferase [predicted]	Bombyx mori	XP_004925677	188	1.07E-57	93.7	24
EP_Unigene_ 14_FAT	GBXH01082860	417	n-acetyltrasnferase 9-like protein [cl17182]	Bombyx mori	XP_004922983	155	1.72E-44	90.2	10
EP_Unigene_ 15_FAT	GBXH01082861	261	n-acetyltrasnferase 2 [predicted]	Bombyx mori	NP_001177771	152	5.12E-44	66.9	0.2
EP_Unigene_ 16_FAT	GBXH01082862	214	Acetyltrasnfease 1 [cl09938]	Agrotis ipsilon	AGQ45622	119	6.17E-31	85.7	9
EP_Contig_4335_FAT	GBXH01004419	496	acetyltransferase 1 [cl09938]	Ostrinia scapulalis	BAH03386	204	8.75E-61	92	113
EP_Contig_ 7673_FAT	GBXH01007751	927	acetyltrasnferase [cl17182]	Agrotis ipsilon	AGQ45625	360	7.31E-122	95	80
EP_Contig_ 18689_FAT	GBXH01018731	2885	n-alpha acetyltransferase [predicted]	Bombyx mori	XP_004922640	1403	0	83.1	3
EP_Contig_ 45366_FAT	GBXH01045245	540	n-acetyltransferase-40like [predicted]	Bombyx mori	XP_004921847	231	5.66E-73	77.55	8
Aldehyde reductase
EP_Unigene_ 1_AR	GBXH01000069	460	Aldo-keto reductase	Agrotis ipsilon	XP_004925119	223	4.10E-69	82.2	30
EP_Unigene_ 2_AR	GBXH01000070	1177	Aldo-keto reductase	Danaus plexippus	EHJ72113	496	9.38E-172	72.5	27
EP_Unigene_ 4_AR	GBXH01000072	846	Aldo-keto reductase	Agrotis ipsilon	AGQ45621	209	9.97E-62	78.3	29
EP_Unigene_ 5_AR	GBXH01000073	1200	Aldo-keto reductase	Chilo suppressalis	AEW46852	487	6.43E-169	73.1	62
EP_Unigene_ 8_AR	GBXH01000074	577	Aldo-keto reductase	Chilo suppressalis	AEW46854	221	9.10E-68	71.5	22
EP_Unigene_ 9_AR	GBXH01000075	470	Aldo-keto reductase	Papilio xanthus	BAM20078	227	1.14E-70	75.7	84
EP_Contig_ 10669_AR	GBXH01010734	263	Aldo-keto reductase	Bombyx mori	XP_004926772	103	2.14E-24	68.9	2
EP_Contig_ 15787_AR	GBXH01015838	1025	Aldo-keto reducase 1	Bombyx mori	XP_004933321	415	6.30E-141	80.6	21
EP_Contig_ 19588_AR	GBXH01019627	244	Aldo-keto reductase	Papilio xuthus	BAM18493	117	2.12E-29	78.6	16
EP_Contig_ 62067_AR	GBXH01061668	400	Aldo-keto reductase	Bombyx mori	XP_004922743	211	3.99E-65	78	0.13
EP_Contig_ 39413_AR	GBXH01039339	205	Aldo-keto reducase domain containing protein	Bombyx mori	XP_004929974	107	7.13E-22	74.3	8

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