Table 4 Comparison of fold change values from RNA-seq data and qRT-PCR in 2Â h LPS and Poly I:C treated BV-2 microglia cells
From: Transcriptome sequencing of microglial cells stimulated with TLR3 and TLR4 ligands
RNA-seq fold change | qRT-PCR fold change | ||||
|---|---|---|---|---|---|
Gene symbol | Gene accession ID | LPS_2h | Poly I:C_2h | LPS_2h | Poly I:C_2h |
Il-1ß | NM_008361 | 93.82 | 96.13 | 89.02 | 96.72 |
Il1A | NM_010554 | 57.04 | 82.69 | 67.98 | 99.02 |
TNF | NM_001278601 | 51.12 | 45.94 | 55.94 | 69.01 |
PTGS2 | NM_011198 | 32.41 | 51.93 | 36.5 | 35.09 |
CXCL10 | NM_021274 | 34.74 | 88.57 | 12.53 | 87.45 |
CCL4 | NM_013652 | 50.02 | 60.01 | 65.23 | 42.25 |
CCL7 | NM_013654 | 17.41 | 44.98 | 52.23 | 35.26 |
CCL3 | NM_011337 | 37.15 | 22.61 | 39.66 | 22.23 |
NFκBIA | NM_010907 | 38.57 | 33.60 | 42.36 | 30.21 |
IRF7 | NM_001252600 | 21.59 | 34.28 | 19.68 | 26.35 |
JUNB | NM_008416 | 20.3 | 22.01 | 22.25 | 22.02 |
IRF1 | NM_001159393 | 14.31 | 26.35 | 16.79 | 18.23 |
CLEC4E | NM_019948 | 53.91 | 53.29 | 47.52 | 45.25 |
GPR84 | NM_030720 | 44.69 | 47.16 | 51.25 | 41.23 |
SLC15A3 | NM_023044 | 18.0 | 16.23 | 22.03 | 11.23 |
KDM4A | NM_001161823 | 23.29 | 18.41 | 19.55 | 16.23 |
KLF7 | NM_033563 | 4.89 | 3.85 | 1.04 | 0.909 |
IRAK3 | NM_028679 | 4.97 | 4.25 | 0.77 | 0.89 |