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Table 5 Comparison of fold change values from RNA-seq data and qRT-PCR in 4 h LPS and Poly (I:C) treated BV-2 microglia cells

From: Transcriptome sequencing of microglial cells stimulated with TLR3 and TLR4 ligands

RNA-seq fold change

qRT-PCR fold change

Gene symbol

Gene accession ID

LPS_4h

Poly I:C_4h

LPS_4h

Poly I:C_4h

Il1ß

NM_008361

98.06

91.24

107.99

84.02

Il1A

NM_010554

66.01

82.29

77.23

96.32

TNF

NM_001278601

57.25

46.18

21.33

50.02

PTGS2

NM_011198

61.7

45.1

42.56

31.02

CXCL10

NM_021274

88.25

79.58

19.64

105.1

CCL4

NM_013652

69.71

61.29

74.25

67.52

CCL7

NM_013654

39.89

68.01

61.66

51.02

CCL3

NM_011337

45.34

30.07

47.85

30.21

NFκBIA

NM_010907

31.46

24.92

39.98

27.45

IRF7

NM_001252600

51.23

54.66

42.25

30.21

JUNB

NM_008416

18.46

16.16

17.58

17.23

IRF1

NM_001159393

17.02

31.08

17.61

26.53

CLEC4E

NM_019948

57.85

57.51

62.03

51.89

GPR84

NM_030720

45.93

45.79

57.89

43.58

SLC15A3

NM_023044

30.12

27.72

27.89

19.86

KDM4A

NM_001161823

17.16

11.20

22.00

12.23

KLF7

NM_033563

4.22

3.24

1.05

0.896

IRAK3

NM_028679

6.99

7.13

1.22

1.102