Table 1 Sequencing, reference mapping and de novo assembly results (as reported by Genewiz Inc.)
Sequencing | Count | |
|---|---|---|
Ethanol Red | ISO12 | |
Total no. of reads | 15,458,851 | 12,449,273 |
Median PHRED score | 38.0 | 38.0 |
Mean GC content | 38 % | 38 % |
Reference mapping to the S288c reference sequence | Count | |
Ethanol Red | ISO12 | |
No. of mapped reads | 15,341,613 (99.2 %) | 12,353,009 (99.2 %) |
Average coverage | 185× | 153× |
De novo assembly | Count | |
Ethanol Red | ISO12 | |
No. of matched reads | 15,390,426 (99.6 %) | 12,195,728 (98.0 %) |
Average read length (bp) | 147.6 | 151.1 |
Total contigs assembled | 218 | 361 |
Total bases in contigs | 11,492,342 | 11,356,874 |
Average coverage | 301× | 196× |
N50 (bp) | 95,106 | 67,943 |