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Table 6 Description of primers used for qPCR validation of differential expression results

From: De novo assembly of a transcriptome from juvenile blue crabs (Callinectes sapidus) following exposure to surrogate Macondo crude oil

Gene (abbreviation)

Trinity Component or Isoform ID

Primer Name

Sequence (5’ to 3’)

Product Size (bp)

Heat Shock Protein 90 (hsp90)

comp22653_c0a

HSP90F2

CACCGACAACATCAAGCTGTAC

93

  

HSP90R2

ACACCACGCACAAAGTTGAG

 

EGL9 (egl9)

comp25287_c2a

EGL9F1

TGTTTTCGGCCTAAGTGGTG

111

  

EGL9R1

AAATCCCGCACTATGCTTGG

 

Hemocyanin

comp16095_c0a

HEMF1

TAACAAGAACCCGGGCAAAG

71

  

HEMR1

AAATGAAGGTCCTGCTGGTG

 

Ribosomal Protein L12 (rpl)

comp18523_c0_seq1

RPNF2

AATCGCAGTTCATCCTCCAC

71

  

RPNR2

GAGGCATGGTGCTGAATTTG

 

Glutamate Oxyacetyl Transaminase 2 (got2)

comp4115_c0_seq1

GOTF2

TTGAGATGATGGGCACTCAG

85

  

GOTR2

TCTTTCAGCATCAGCACCAG

 

Short-chain Dehydrogenase Reductase (sdr1)

comp25500_c1_seq1

SDRF1

GTGCCGTAGTCATTATTCTCAGC

78

  

SDRR1

TGTGGTGGTTTGACATGCAC

 

sdr1-alternatively spliced variant (sdr1-ASV)

comp25500_c1_seq2

SDRF3

CTTTCACTGCGACAAAAATCGG

136

  

SDRR3

CCTTGTTAACGAACCACCACTG

 

Glucuronosyltransferase (ugt1)

comp16829_c0_seq2

UGTF2

AGCCAAGCAGGATGAGACTAAG

76

  

UGTR2

TCTTGCATCAACACCAACGC

 

ugt1-alternatively spliced variant (ugt1-ASV)

comp19629_c0_seq1

UGTF3

CAGTGGATGTGCAAATAATCGTC

116

  

UGTR3

TGGGGCATCAAAGTACTGGTAG

 
  1. aComponent consists of multiple transcripts that are all amplified by the primer pair