De novo assembly of a transcriptome from juvenile blue crabs (Callinectes sapidus) following exposure to surrogate Macondo crude oil

Table 7 qPCR results for treatment and control individuals

Gene ID	Trinity Isoform ID	Individual	Relative Quantitation (Log₂)
rpl	comp18523_c0_seq1	C1	−0.539
		C2	−0.150
		C3	0.806
		T1	0.030
		T2	−0.037
		T3	0.363
		T4	−0.202
got2	comp4115_c0_seq1	C1	0.619
		C2	0.294
		C3	−0.796
		T1	0.075
		T2	0.142
		T3	−0.258
		T4	0.306
ugt1	comp16829_c0_seq2	C1	−2.591
		C2	3.790
		C3	0.874
		T1	−0.211
		T2	1.367
		T3	1.516
		T4	2.573
sdr1	comp25500_c1_seq1	C1	−1.304
		C2	2.329
		C3	−0.151
		T1	−0.068
		T2	0.730
		T3	1.834
		T4	−4.589
hsp90	comp22653_c0_seq_1 & 2	C1	−4.509
		C2	3.421
		C3	3.737
		T1	−0.878
		T2	−2.614
		T3	−1.886
		T4	−2.239
egl9	comp25287_c2_seq_1, 2, & 3	C1	−4.053
		C2	2.790
		C3	3.581
		T1	−1.034
		T2	3.922
		T3	5.808
		T4	2.946
hemocyanin	comp16095_c0_seq1 & 2	C1	7.418
		C2	−0.337
		C3	−1.645
		T1	−5.403
		T2	9.374
		T3	9.420
		T4	1.106

Relative quantitation values for control and treatment individuals for validated genes and transcripts. C1-C3 = control group; T1-T4 = treatment/oil group

ISSN: 1471-2164