Fig. 1

Immune genes expression at different time points upon Pam3CSK4 stimulation. Embryos were injected at 27 hpf with 1 ng Pam3CSK4 and expression levels of il1b (a), il8 (b), tnfa (c), il10 (d) and il6 (e) were determined at 1, 3 and 6 hpi by qPCR. Data (mean ± SEM) are combined from at least three biological replicates (n = 15 embryos per group) and expressed relative to their corresponding mock injection (water) control, which is set at 1. Statistical significance of differences between mock and Pam3CSK4 groups was determined by ANOVA analysis and Tukey’s multiple comparisons test, *p < 0.05, **p < 0.01, ***p < 0.001