Fig. 9

Verification of the relative expression levels of genes by quantitative RT-PCR (qRT-PCR). a Correlation of the expression levels of 45 randomly selected genes measured by qRT-PCR and RNA-seq. b Expression patterns of 16 unigenes involved in the flavonoid, caffeine, and theanine biosynthetic pathways by qRT-PCR (Red bar) and RNA-seq (Blue line). DFR, dihydroflavonol 4-reductase; LDOX, leucoanthocyanidin oxidase; TCS, tea caffeine synthase; ANR, anthocyanidin reductase; IMPDH, IMP dehydrogenase; GS, glutamine synthetase; Anase, adenosine nucleosidase; F3H, flavanone 3-hydroxylase; CHS, chalcone synthase; CHI, chalcone isomerase; FLS, flavonol synthase; TS, theanine synthetase; F3′H, flavonoid 3′-hydroxylase; GLS, glutaminase; ADC, arginine decarboxylase. c Expression patterns of 14 unigenes that were specifically expressed in second leaf according to the RNA-seq results and in first leaf, second leaf, and two and a bud according to the qRT-PCR (Red bar) and RNA-seq (Blue line) results