Table 1 Characteristics of the genes selected for the pooled and individual RT-qPCR analyses. The proteins, primers and efficiencies values are indicated. The genes selected for the individual analysis are indicated in bold
Gene code | Protein | Primers | Efficiencies % | Genbank accession # |
|---|---|---|---|---|
KRMP | Lysine (K)-rich mantle protein | Joubert et al. 2014 | 95 | JZ845792 |
SHEM 9B | Shematrin 9 | A_shem9b 5’- F CCCCGTATCCTCCATATCC | 101 | JZ845577 |
A_shem9b 3’- R GCTATTACCGGAGTACCCTACG | ||||
SHEM 1B | Shematrin 1 | C_Shem1b F 5’- CGCTATCGTTGCTCTCATTG | 101.00 | JZ845578 |
C_Shem1b R 3’- TCCACCTCCTCCTCCTCTTC | ||||
TYR 2A | Tyrosinase 2 | A_tyr2a F 5’- GCGGCTCTACTGTCAAATGG | 112.00 | JZ845579 |
A_tyr2b R 3’- CTGGACCTTTCAGGGACTGG | ||||
TYR 2C | Tyrosinase 2 | A tyr2c F 5’- CCCGTGGCCTGGATAGTC | 101.00 | JZ845580 |
A tyr2c R 3’- TTTTCTTCCATCACTGCTACATTG | ||||
FLAV | Flavonol cinnamoyl CoA reductase-related | A_flav aF 5’- AGCAGGGTTATCACGTCAGG | 96.00 | JZ845581 |
A_flav bR 3’- ATCCTTTGGTGCCTGTGC | ||||
METH | Methionine-rich nacre protein | A_Meth bF 5’- ATGCGGAGGATACTGTGCTT | 100.00 | JZ845582 |
A_Meth bR 3’- CGGGGCTGGATAGACTCATA | ||||
CLP3 | Chitinase 3 protein | A_clp3b F 5’- TCACAAAATGGATCATAACGTACC | 94.00 | JZ845583 |
A_clp3b R 3’- GGACTGCCTTTGAATGTCG | ||||
PDZ | PDZ domain protein | A_PDZa F 5’- TGAGCTTCAGAGAGGTGACG | 100.00 | JZ845584 |
A_PDZa R 3’- AACATTTGGTGAGGGTTTGG | ||||
MP10 | Mantle protein 10 | A_MP10d F 5’- ATTATGGACCGGGCAAGC | 100.00 | JZ845585 |
A_MP10c R 3’- GAGGACAGGAACATCAACAGG | ||||
EGF | Epidermal growth factor receptor | A_EGF aF 5’- CATTCCCCATCTTCCTCAAA | 108.00 | JZ845586 |
A-EGF aR 3’- GGACTTCCTGGGATGTTGTC | ||||
PHOS | Phosphotyrosine protein phosphatase | A_Phos bF 5’- AATTATGACACAGAGGGATCTAAGG | 98.00 | JZ845587 |
A_Phos aR 3’- CATAGAAACCACCACAACATCG | ||||
YTH | YTH domain family protein 2-like | A_YTH aF 5’- AATTGAGACACATTCGGTTGG | 93.00 | JZ845588 |
A_YTH aR 3’- TTACCGCTCTCTGTCCTTGC | ||||
PIF | Pif177-like protein | A_pif aF 5’- TTTTGAATTACACGACTGCTTTG | 93.00 | JZ845589 |
A_pif aR 3’- TTCAGTAGAACTAACGCTAAATCCAG | ||||
CHIT | Chitin synthase | A_Chit aF 5’- AATCCAATTTCCCGCAGTC | 90.00 | JZ845590 |
A_Chit bR 3’- TTGTTGTAGACATTAGCGACGTATC | ||||
YBOX | Y-box binding protein | A_Ybox aF 5’- CGTACATCAAACTGCCATTACC | 97.00 | JZ845591 |
A_Ybox aR 3’- CTACATCAAATTCCACCTTCTCC | ||||
COLL | Collagen alpha-1 (XI) chain precursor | A_Coll bF 5’- CGTCCAATCAATCCAGGTG | 105.00 | JZ845592 |
A_Coll aR 3’- TGGGTCCAAAAGGTGAAATG | ||||
GIGA | Gigasin | A_Giga aF 5’- TCTCTTCCGGTCAAAAATGC | 91.00 | JZ845593 |
A_Giga aR 3’- TCGCTGTACTATTTCCGTTCG | ||||
SHEM 4 | Shematrin 4 | A_Shem4a F 5’- GCTTCCCATCGGTTTATGG | 92.00 | JZ845594 |
A_Shem4b 3’- R TGCCAACATTTCCGTATCC | ||||
CAL | Calreticulin | A_Cal aF 5’- TCACCATCCATTTCATCATCC | 102.00 | JZ845595 |
A_Cal aR 3’- ACCAGAGGACTGGGACAAGC | ||||
PEROX | Peroxidase | A_Perox aF 5’- TGCTGGGACTCACTCTATCCA | 107.00 | JZ845596 |
A_Perox bR 3’- TCAAGCCATCAAAGAAACATCTT | ||||
MP8 | Mantle protein 8 | Joubert et al. 2014 | 101.00 | JZ845791 |
ASP | Aspein shell matrix protein | Joubert et al. 2014 | 99.00 | JZ845790 |
MAT | Matrilin Cartilage matrix protein | A_Mat aF 5’- TTGTTCTGGATGGGTCTTCG | 109.00 | JZ845597 |
A_Mat aR 3’- TCAAAGCCCGCACATCAG | ||||
SHEM 8 | Shematrin 8 | C_Shem8 bF 5’- CTCCACCACCAATGACGATT | 111.00 | JZ845598 |
C_Shem8 aR 3’- TTTCGGGGGTGTTAACGTAG | ||||
FER | Ferritin | C_Fer cF 5’- GGGCCTGATTGACAGACTTCT | 96.00 | JZ845599 |
C_Fer cR 3’- GAGGGCGCATTGTCCTTC | ||||
PERL | Perlin matrix protein | C_Perl bF 5’- TTCCAGATATTACACCCTGTGCT | 100.00 | JZ845600 |
C_Perl aR 3’- CGTTACCGTTTCCACCAAAA | ||||
PRISM | Prism uncharacterized shell protein | C_Prism aF 5’- TGGTTCCAAGTGTATTTGTCCA | 96.00 | JZ845601 |
C_Prism aR 3’- TCCATAGACGCACACCTTTG | ||||
MP88 | molluscan prismatic and nacreous layer 88 | C_MP88a F 5’- TCTGTGAAAATTTTGATAAACTGAA | 109.00 | JZ845602 |
C_MP88a R 3’- GAATAAAAGTTTAATGTTCCATTCCT | ||||
CHK1 | Checkpoint-like protein | C_CHK1a F 5’- CGGGCAGGTACTCATTCC | 95.00 | JZ845603 |
C_CHK1b R 3’- GCTACCACATCCAAGGAAGG | ||||
LAMIN | Laminin receptor precursor | C_Lamin bF 5’- GAAGCTGAGAAAGAAGAACAGACC | 101.00 | JZ845604 |
C_Lamin aR 3’- TGTTGTGGTGGCTGTATTGC | ||||
SEST | Sestrin 1-like protein | C_Sest aF 5’- TTTTTGCTACCAGGACTTTGC | 91.00 | JZ845605 |
C_Sest aR 3’- GTGTCAACATCTGTCATCTCACC | ||||
FIBRO | Fibronectin 3 | C_Fibro aF 5’- AGTAGCTGATCGTGCATTCG | 97.00 | JZ845606 |
C_Fibro bR 3’- AACTCACAGGACACCAAAACG | ||||
SHELL | Nacre uncharacterize shell protein 6 | C_Shell_cF 5’- CGTTCTATCCCTGGTCATCC | 101.00 | JZ845607 |
C_Shell_dR 3’- GGACGTGGATTTTCCTTGG | ||||
SERP | Serine protease inhibitor | C_SerP_cF 5’- AGGTGTGTACCATTCTTCTACGG | 92.00 | JZ845608 |
C_SerP_cR 3’- GCAAACATCTCCTCCATCTCC | ||||
ZINC | Zinc metalloprotease | C_Zinc bF 5’- CAGAGATGGTTTTGTGTTACTTACG | 105.00 | JZ845609 |
C_Zinc aR 3’- GCTTTGAGGCATTCATGTCC | ||||
TRANS | Translocon-associated protein subunit gamma-like | C_Trans aF 5’- TGCCCTAAAGAGGGAAAGC | 102.00 | JZ845610 |
C_Trans aR 3’- CCACAATTAGCAGCACAAGG |