Fig. 7

Domain analysis and sequence characteristics of the IFITM1, 2 and 3 gene family. The sequence alignment of chicken (GALGA), duck (ANAPL) and human (HOMSA) IFITM peptides was created using T-coffee and displayed using Jalview, which was also used to create secondary structure (JNETHMM; red, helices; green, beta sheets) and conservation tracks. Sites predicted to be under positive selection (P_SITES, Table 1) are shown as triangles (red, duck and yellow, other birds). The transmembrane (TM1 and TM2), conserved intracellular loop (CIL) and other domains were predicted using SMART, SOSUI and ExPasy. MOTIFS, mark amino acid residues as triangles and are discussed in the main text: yellow Cysteine, green Phenylalanine, purple Lysine. Sequence logos were generated using Weblogo and based on the alignment of the duck, chicken and human IFITM sequences. For details of methods see Materials and Methods