Identification of promising host-induced silencing targets among genes preferentially transcribed in haustoria of Puccinia

Table 1 Summary of ten Puccinia graminis f. sp. tritici genes contributing to pathogenicity or development

Gene ID	Putative gene functions	Transcript levels^a	SP^b	Homolog similarity (nt identities %)		Homolog similarity (aa identities %)		Frequency of low ITs^c
Gene ID	Putative gene functions	Haust/Infw	SP^b	Pt	Pst	Pt	Pst	Frequency of low ITs^c
PGTG_01136	Fructose-bisphosphate aldose	5.80	No	89	86	91	81	14/164
PGTG_01215	Glycoside hydrolase family 26	5.8	Yes	83	83	80	80	22/131
PGTG_03478	Glycoside hydrolase family 76	7.3	Yes	N/A	N/A	52	53	9/143
PGTG_14350	Transporter component-like	2.4	Yes	86	82	89	88	25/154
PGTG_10731	Predicted protein	6.6	No	85	81	91	85	22/132
PGTG_12890	Predicted protein	7.1	No	78	81	82	80	32/160
PGTG_01304	Thiazole biosynthetic enzyme	2.1	No	91	88	95	96	23/127
PGTG_16914	Amino acid transporter	15.4	No	86	85	83	83	7/69
PGTG_03590	Small secreted protein	81.8	Yes	N/A	N/A	N/A	N/A	15/159
Pgt-IaaM	Tryptophan 2-monooxygenase	11.5	No	80	83	82	79	13/141

^aFrequency of fungal RNA sequence reads for the transcript in isolated haustoria of infected plants over the frequency from RNA from infected leaves
^b SP predicted signal peptide
^cNumber of seedlings showing reduced infection types/number of seedlings used in VIGS assays. Data are pooled from four or more experiments

ISSN: 1471-2164