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Table 4 Sanger sequencing validation comparison on variant subsets of TargetSeq-Proton and SureSelect-HiSeq data calls

From: Comparison and evaluation of two exome capture kits and sequencing platforms for variant calling

 

SureSelect-HiSeq-specific

TargetSeq-Proton-specific

Concordant

 

1-bp InDels

SNPs

1-bp InDels

SNPs

1-bp InDels

SNPs

Validated true

89.6 %(60)

88.3 %(53)

15.8 %(6)

60.0 %(21)

100.0 %(47)

91.5 %(65)

Validated false

10.4 %(7)

11.7 %(7)

84.2 %(32)

40.0 %(14)

0.0 %(0)

8.2 %(6)

  1. A total of 240 SNPs and 240 1-bp InDels from four samples were randomly selected for Sanger sequencing validation, with 80 loci from the set of TargetSeq-Proton-specific, 80 from the set of SureSelect-HiSeq-specific, and 80 from the set of concordance between two platforms
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BMC Genomics

ISSN: 1471-2164

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