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Table 4 Sanger sequencing validation comparison on variant subsets of TargetSeq-Proton and SureSelect-HiSeq data calls

From: Comparison and evaluation of two exome capture kits and sequencing platforms for variant calling

  SureSelect-HiSeq-specific TargetSeq-Proton-specific Concordant
  1-bp InDels SNPs 1-bp InDels SNPs 1-bp InDels SNPs
Validated true 89.6 %(60) 88.3 %(53) 15.8 %(6) 60.0 %(21) 100.0 %(47) 91.5 %(65)
Validated false 10.4 %(7) 11.7 %(7) 84.2 %(32) 40.0 %(14) 0.0 %(0) 8.2 %(6)
  1. A total of 240 SNPs and 240 1-bp InDels from four samples were randomly selected for Sanger sequencing validation, with 80 loci from the set of TargetSeq-Proton-specific, 80 from the set of SureSelect-HiSeq-specific, and 80 from the set of concordance between two platforms