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Table 1 Properties of Cryptosporidium oocyst and DNA preparations from two pilot phase stool samples

From: Generation of whole genome sequences of new Cryptosporidium hominis and Cryptosporidium parvum isolates directly from stool samples

Isolate, species and gp60 subtype Preparation stage qPCR 18S rRNA gene (CT) from original stool sample Quantification of Cryptosporidium processed by IMS Bacterial DNA calculated from qPCR 16 s rRNA gene (ng) (% reduction compared with salt-floated suspension) Total DNA from Qubit (ng)
  Numbers of oocysts counted; genome copies derived from microscopy counts Genome copies calculated from qPCR 18 s rRNA gene   
UKP2, C. parvum IIaA19G1R2 Salt-floated suspension 28.8 1.0 ×106; 4.0×106 4.27×105 323.1 ND
Post IMS, bleach treated suspension   3.7×105; 1.5×106 2.99×105 4.2 (98.7 % reduction) 2.44
Post CsCl, bleach treated suspension   1.3×105; 5.2 ×105 1.99×105 NDa 1.52
UKH3, C. hominis IbA10G2 Initial suspension 26.7 1.0×106; 4.0×106 4.6×105 86.7 ND
Post IMS, bleach treated suspension   2.9×105; 1.2×106 1.3×106 4.4 (94.9 % reduction) 2.68
  1. ND, not done
  2. aNot tested in order to preserve DNA as total DNA level was low