Table 1 Properties of Cryptosporidium oocyst and DNA preparations from two pilot phase stool samples
Isolate, species and gp60 subtype | Preparation stage | qPCR 18S rRNA gene (CT) from original stool sample | Quantification of Cryptosporidium processed by IMS | Bacterial DNA calculated from qPCR 16Â s rRNA gene (ng) (% reduction compared with salt-floated suspension) | Total DNA from Qubit (ng) | |
|---|---|---|---|---|---|---|
| Â | Numbers of oocysts counted; genome copies derived from microscopy counts | Genome copies calculated from qPCR 18Â s rRNA gene | Â | Â | ||
UKP2, C. parvum IIaA19G1R2 | Salt-floated suspension | 28.8 | 1.0 ×106; 4.0×106 | 4.27×105 | 323.1 | ND |
Post IMS, bleach treated suspension |  | 3.7×105; 1.5×106 | 2.99×105 | 4.2 (98.7 % reduction) | 2.44 | |
Post CsCl, bleach treated suspension |  | 1.3×105; 5.2 ×105 | 1.99×105 | NDa | 1.52 | |
UKH3, C. hominis IbA10G2 | Initial suspension | 26.7 | 1.0×106; 4.0×106 | 4.6×105 | 86.7 | ND |
Post IMS, bleach treated suspension |  | 2.9×105; 1.2×106 | 1.3×106 | 4.4 (94.9 % reduction) | 2.68 | |