Fig. 2
From: Reducing amplification artifacts in high multiplex amplicon sequencing by using molecular barcodes
Comparison of sensitivity and false-positive rates for different input DNA amounts. (a and b) The x-axis represents different input quantity of the DNA admixture. The left y-axis represents detection sensitivity for SNVs at 1–2 % fraction. The right y-axis represents false positive rates (a) Performance using the original protocol. (b) The sensitivity of SNV detection was significantly higher after adding 3 cycles of limited amplification. (c) The ROC curve from 80 ng 3-cycle data with or without using the information of molecular barcodes