Table 2 Summary of the sequencing runs for FFPE samples
From: Reducing amplification artifacts in high multiplex amplicon sequencing by using molecular barcodes
Sample ID | T5 | LN2 | LT2 | T2 |
|---|---|---|---|---|
Total reads | 1,053,646 | 11,352,414 | 13,518,788 | 9,911,538 |
On-target reads | 1,015,755 | 11,027,934 | 13,034,688 | 9,642,483 |
On-target read pairs | 501,517 | 5,417,637 | 6,346,625 | 4,745,809 |
Median read depth | 820 | 9,653 | 11,460 | 8,420 |
Mean read depth | 1,079 | 11,534 | 13,526 | 10,120 |
% Bases >0.2x mean depth | 90 | 93 | 94 | 93 |
Median consensus read depth | 30 | 390 | 908 | 151 |
Mean consensus read depth | 33 | 376 | 891 | 146 |
Mean raw read/consensus read | 15 | 14 | 8 | 33 |
Median raw read/consensus read | 15 | 13 | 7 | 32 |
Bases in target region | 86,544 | 86,544 | 86,544 | 86,544 |
Called SNVs | 77 | 129 | 134 | 141 |