Fig. 3

Transcript and CHART analysis of xylanase-encoding genes in the presence or absence of Xyr1. The T. reesei wild-type strain (dots) and the Δxyr1-strain (triangles) were pre-grown on glycerol and thereafter incubated on D-glucose (G), D-xylose (X) or sophorose (S) for 3 h. The core promoter region (red) and an URR (blue) of xyn1 (a) and xyn2 (b) genes were investigated. The gene expression analysis was performed by cDNA synthesis followed by qPCR, and transcript levels are depicted on the x-axis. CHART-PCR was performed by DNaseI digestion followed by qPCR, and CAIs are depicted on the y-axis. In both cases sar1 and act genes were used for data normalization and the wild-type strain incubated without carbon source for 3 h was the reference condition. The dashed line indicates transcript level of the reference condition, i.e. levels above are considered induced and levels below are considered repressed. All values are means from measurements in triplicates and three biological experiments (cultivations). The error bars indicate standard deviations. Diagrams are identically scaled