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Fig. 4 | BMC Genomics

Fig. 4

From: The impact of chromatin remodelling on cellulase expression in Trichoderma reesei

Fig. 4

CHART analysis of cellulase- and xylanase-encoding genes in presence and. The T. reesei wild-type strain (blue bars) and the Δxyr1-strain (red bars) were pregrown on glycerol and thereafter incubated without carbon source (N) or in presence of 2.0 mM sophorose (S) for 3 h. The core promoter regions of cbh1, cbh2, xyn1, and xyn2 genes were investigated. CHART-PCR was performed by DNaseI digestion followed by qPCR using sar1 and act genes were for data normalization. Chromatin accession indices (CAI) are depicted on the y-axis. All values are means from measurements in triplicate and three biological experiments (cultivations). The error bars depict the standard deviation and different letters denote statistical difference among compared data employing t-test (P < 0.05)

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