Fig. 5

In vivo footprinting analyses of the cbh1 promoter in the presence or absence of Xyr1. The T. reesei wild-type strain QM6a and the Δxyr1-strain were pre-grown on glycerol and then incubated on D-glucose or sophorose for 3 h followed by DMS-induced in vivo methylation. a Schematic drawing of the cbh1 promoter and the investigated regions (indicated by green lines). Two URRs (b, c) bearing functional Xyr1-binding sites (orange) or Cre1-sites (purple) and the core promoter region (d) bearing Xyr1-binding sites (orange) were investigated on the forward strand. Numbers indicate the position of the base upstream from ATG. Analysis of data and visualization was performed using ivFAST [34]. Only signals that are statistically different are considered; protected bases are highlighted in red shades and hypersensitive bases are highlighted in blue shades; the three colour intensities each correspond to stronger differences between compared conditions (Δxyr1-strain compared to wild-type strain), i.e. increasing colour intensity means more than 1.1-, 1.3-, and 1.5-fold difference