Fig. 3

Immunolocalisation of salmon louse aquaporins expressed in X. laevis oocytes. a Immunofluorescence photomicrographs of paraffin sections of water- (Ctrl) and salmon louse cRNA-injected oocytes probed with paralog-specific antisera followed by Cy3-labeled anti-rabbit IgG. Arrows point to the plasma membrane. Sections shown are representative of five different sections per treatment showing identical staining. b Representative immunoblots of total oocyte membranes (0.5-3 oocyte equivalents per lane) with molecular markers shown to the left. Arrows point to bands that match the predicted molecular mass of each aquaporin paralog, while lower bands likely repesent misfolded and degraded proteins. For Aqp12L2 a cross-reaction is seen against a ~45 kD X. laevis protein