Fig. 7
From: A clone-free, single molecule map of the domestic cow (Bos taurus) genome
Examples of large-scale map vs. sequence discordances (IT discordances). a: GPS (Methods) viewer shows the BtOM1.0_chr1 optical map (center track) alignments to UMD3.1_chr1 and Btau4.6_chr1 revealing 759 Kb translocated region (UMD3.1) and a 1,284 Kb inversion (Btau4.6). Color scheme is the same as Fig. 5, with cyan highlighting BtOM1.0_chr1 portions aligning to UMD3.1_chr1 or Btu4.6_chr1. b: ~1 Mb region of chromosome 17: the in silico maps (blue track) of UMD3.1_chr17 perfectly align to the BtOM1.0_chr17, but Btau4.6_chr17/BtOM1.0_chr17 alignment reveals two misassembled regions in the Btau4.6 sequence build: an inversion (blue and gold arrows) and extraneous sequence. Restriction fragments (undulating boxes) are color keyed as: gold (agreement with blue track, or reference); red (extra cuts in optical map); cyan (missing cuts in optical map); and purple (compound events). c: ~500 Kb region on chromosome 19: alignment of BtOM1.0_chr19 and Btau4.6_chr19 (blue track) suggest that the Btau4.6_chr19 sequence (blue arrow) was probably misplaced and should be inverted and placed nearby. BtOM1.0_chr19 and UMD3.1_chr19 (blue track) alignment suggests that sequence here also presents misassemblies similar to Btau4.6_chr19