Fig. 5

CpG coverage and methylation status at the 12th position of the CTCF motifs. a CpG coverage (%) distribution within regions [−50 bp, +50 bp] of the center of CTCF-A and CTCF-C binding sites in GM12878 (see Additional file 10: Figure S5 for K562 and HeLaS3). See Additional file 11: Figure S6 for the corresponding DNA methylation distribution. b Depletion of cohesin (represented by overlapping binding peaks of SMC3 and Rad21) at CTCF binding sites. The black and gray bars represent cohesin coverage at all CTCF binding regions and at CTCF binding sites that are methylated (methylation level >20 %) at the 12th position, respectively. c Correlation (Pearson’s correlation coefficients) between the ChIP-seq read counts for cohesin (Rad21) and the DNA methylation level at the 12th position of the CTCF binding sites across the three cell lines. The stars (*) indicate the statistical significance in each test (***, p-value < 1E-5, **, p-value < 1E-2, * p-value < 5E-2)