Fig. 3
From: Polyglutamine toxicity in yeast induces metabolic alterations and mitochondrial defects
Effect of different polyQ stretches in yeast cells on phosphate metabolism. a 31P-NMR spectra of yeast cells expressing Q0-YFP (top), Q30-YFP (middle) or Q56-YFP (bottom). Yeasts were resuspended to an OD595 = 300 in phosphate free buffer. Chemical shift positions are listed in Additional file 7, 1: inorganic phosphate, 2: polyphosphate (end of chain), 3: polyphosphate (internal). b Exophosphatase activity was monitored upon transferring yeasts to phosphate free media. Q0-YFP is depicted in black, Q30-YFP in blue and Q56-YFP in red. Results were obtained from three independent experiments. Values were normalized for better comparability. Mean and SEM are depicted. Statistical significance is indicated with a star (α = 0.05)