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Table 1 Summary of sequencing results. Number of sequenced, processed and aligned reads

From: MicroRNAs in the oriental fruit fly, Bactrocera dorsalis: extending Drosophilid miRNA conservation to the Tephritidae

Library

Total number of sequenced reads

Total usable reads equal to or longer than 17 nt

Total reads mapped

Number of unique sequences

Number of unique sequences mapping to at least one and up to 4 locations in the genome

Unique sequences matching an Rfam model (non-coding RNA)

Eggs (rep1)

661,329

649,691

153,904

168,959

46,142 (27.31 %)

3,471 (2.05 %)

Eggs (rep2)

1,783,633

1,755,385

391,420

415,781

114,655 (27.58 %)

6,723 (1.61 %)

Eggs (rep3)

1,511,629

1,495,468

274,923

339,551

97,218 (28.63 %)

5,117 (1.51 %)

Larvae (rep1)

990,551

901,206

181,715

199,654

55,738 (27.92 %

5,156 (2.58 %)

Larvae (rep2)

1,382,185

1,286,889

181,896

303,152

71,141 (23.47 %)

4,645 (1.53 %)

Larvae (rep3)

730,458

697,460

102,637

172,540

41,204 (23.88 %)

3,452 (2.00 %)

Male Pupae (rep1)

1,659,261

1,502,737

237,960

258,875

39,084 (15.10 %)

4,298 (1.66 %)

Male Pupae (rep2)

1,175,996

1,070,491

112,938

164,853

23,601 (14.32 %)

2,988 (1.81 %)

Male Pupae (rep3)

1,253,417

1,206,485

178,091

176,704

25,543 (14.46 %)

3,472 (1.96 %)

Female Pupae (rep1)

1,086,136

1,017,418

115,176

174,118

19,855 (11.40 %)

2,501 (1.43 %)

Female Pupae (rep2)

--

--

--

--

--

--

Female Pupae (rep3)

1,169,052

1,096,264

169,583

153,575

23,841 (15.52 %)

3,239 (2.1 %)

Ovaries (rep1)

79,391

73,001

10,453

18,251

3,270 (17.92 %)

598 (3.27 %)

Ovaries (rep2)

1,154,349

1,134,024

229,095

398,853

111,069 (27.85 %

6,114 (1.53 %)

Ovaries (rep3)

1,740,634

1,710,835

358,303

523,507

145,910 (27.87 %)

8,025 (1.53 %)

TOTAL

16,378,021

15,597,354

  

818,271

59,799

(23.60 % of unique reads)

(27 % of unique reads)