Fig. 3

Colletotrichum lentis ClCE8 homologs are conserved in fungal pathogens. a. Six ClCE8 (JK998676) homologs are identified in the NCBI non-redundant protein database using a stringent E value (1e-50). Csfas, Colletotrichum sublineola (KDN70693); Cffas, Colletotrichum fioriniae (XP_007601018); Cgramfas, Colletotrichum graminicola (EFQ34995); Cglofas, Colletotrichum gloeosporioides (EQB59239); Chfas, Colletotrichum higginsianum (CCF47579); and Cofas, Colletotrichum orbiculare (ENH86023). Peptide sequences were aligned using ESPrint version 3.0. Putative signal peptides (signature characteristic of effectors) were predicted using SignalP version 4.1. Signal peptide and fasciclin domains (pfam02469) are over-lined with black and green colors, respectively. b. A Neighbor-Joining phylogram of ClCE8 homologs. The Arabidopsis thaliana fasciclin-like arabinogalactan protein (Atfas, NP_566398) was used as an outgroup. The evolutionary distance was estimated using a Poisson-correction model and is in the units of the number of amino acid substitutions per site. Values at nodes indicate the percentage of branch support derived from 1000 bootstrap replicates