Fig. 2
Summary of Transcriptomic Profiles. a RMA normalized intensity values for genes with the highest variance across all samples (variance > 2.0) were visualized; clear sex-specific and treatment-specific (TCDD or corn oil) abundance patterns were observed. Intensity values for each sample were clustered with the DIANA hierarchical clustering algorithm, with Pearson’s correlation as a similarity metric. Shading (white to blue) represents RMA normalized intensity values. b Linear modeling was performed to identify differences between treatment and control groups. Each sex was evaluated separately and results combined after modeling was applied. The legend indicates the experimental (i.e., M125 = male TCDD treated mice (125 μg/kg) relative to male vehicle control animals). The number of genes determined to be significantly altered at a range of FDR-adjusted p-value thresholds were examined across experimental groups. c Results (log2 fold-change) were compared between male and female mice for each dosage group. Pearson’s correlation indicated increasing similarity between transcriptomic profiles of male and female mice as TCDD dose was increased. Overlap of significantly altered genes following each dose of TCDD in (d) male and (e) female cohorts